Fosb^tm3.1Yun
Targeted Allele Detail

Summary

• Symbol: Fosb^tm3.1Yun
• Name: FBJ osteosarcoma oncogene B; targeted mutation 3.1, Yusaku Nakabeppu
• MGI ID: MGI:5634958
• Synonyms: fosB^F
• Gene: Fosb Location: Chr7:19036621-19043970 bp, - strand Genetic Position: Chr7, 9.56 cM, cytoband A2-B1
• Alliance: Fosb^tm3.1Yun page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211298
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutation: Nucleotide substitutions
• Mutation details: Three bases (GAGGTGAGA->GAAGTTCGA) in the alternative splicing donor site and two bases (AGTGAA->TCTGAA) in the splice acceptor site were modified via homologous recombination to prevent expression of the long transcript while retaining expression of the shorter transcript. A floxed neo cassette was also inserted in intron 3. Cre mediated recombination removed the neo cassette. This allele lacks expression of the deltaFosb isoform. (J:220449)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fosb Mutation: 22 strains or lines available

References

• Original: J:220449 Ohnishi YN, et al., Antagonistic regulation of cell-matrix adhesion by FosB and DeltaFosB/Delta2DeltaFosB encoded by alternatively spliced forms of fosB transcripts. Mol Biol Cell. 2008 Nov;19(11):4717-29
• All: 2 reference(s)