Actb^{tm1.1(tetO-COP4*/EYFP)Ahky}
Targeted Allele Detail

Summary

• Symbol: Actb^{tm1.1(tetO-COP4*/EYFP)Ahky}
• Name: actin, beta; targeted mutation 1.1, Akihiro Yamanaka
• MGI ID: MGI:5634281
• Synonyms: TetO ChR2
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^{tm1.1(tetO-COP4*/EYFP)Ahky} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211265
• Parent Cell Line: EGR-G01 (ES Cell)
• Strain of Origin: (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: An expression cassette containing a tetracycline responsive element that drives inducible expression of a channelrhodopsin-2 (with an E123T/T159C mutation) and EYFP fusion was inserted 800 bp downstream of the endogenous polyadenylation sequence. Flp-mediated recombination removed an FRT-flanked neo cassette. (J:211265)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 55 strains or lines available

Notes

EGR-G01 cells carry one copy of Tg(CAG-EGFP,Acr-EGFP)2Osb.

References

• Original: J:211265 Tsunematsu T, et al., Optogenetic manipulation of activity and temporally controlled cell-specific ablation reveal a role for MCH neurons in sleep/wake regulation. J Neurosci. 2014 May 14;34(20):6896-909
• All: 4 reference(s)