Col1a1^{tm4(tetO-Pou5f1,-Klf4,-Sox2,-Myc)Yasu}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm4(tetO-Pou5f1,-Klf4,-Sox2,-Myc)Yasu}
• Name: collagen, type I, alpha 1; targeted mutation 4, Yasuhiro Yamada
• MGI ID: MGI:5621181
• Synonyms: Col1a1::tetO-OSKM-ires-mCherry, Col1a1^{tm4(tetO-OSKM,-mCherry)Yasu}, OSKM-inducible
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm4(tetO-Pou5f1,-Klf4,-Sox2,-Myc)Yasu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212039
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm4(tetO-Pou5f1,-Klf4,-Sox2,-Myc)Yasu} expresses 4 genes
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Klf4 (MGI:1342287)
  mouse	Myc (MGI:97250)
  mouse	Pou5f1 (MGI:101893)
  mouse	Sox2 (MGI:98364)

• Mutation details: The tetOP-OKSM "flip-in plasmid" contained a splice acceptor-double polyA sequence and the tetracycline responsive element (TRE, tetOP, or tetO) upstream of the four mouse reprogramming genes Oct4 (Pou5f1; POU domain, class 5, transcription factor 1), Klf4 (Kruppel-like factor 4 (gut)), Sox2 (SRY-box containing gene 2), and c-Myc (Myc; myelocytomatosis oncogene) separated by three different 2A sequences that mediate ribosomal skipping (F2A [from foot-and-mouth disease virus], IRES [internal ribosome entry site], and E2A [from equine rhinitis A virus], respectively) and followed by an IRES-mCherry. (J:212039)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm2(tetO-Pou5f1)Jae} (J:159351).

References

• Original: J:212039 Ohnishi K, et al., Premature termination of reprogramming in vivo leads to cancer development through altered epigenetic regulation. Cell. 2014 Feb 13;156(4):663-77
• All: 4 reference(s)