Panx1^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Panx1^{tm1d(KOMP)Wtsi}
• Name: pannexin 1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5616343
• Gene: Panx1 Location: Chr9:14917081-14956774 bp, - strand Genetic Position: Chr9, 4.52 cM
• Alliance: Panx1^{tm1d(KOMP)Wtsi} page
• IMPC: Panx1 gene page

Mutation origin

• Mutant Cell Line: EPD0309_3_B01
• Germline Transmission: Earliest citation of germline transmission: J:208888
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 14921064 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 14921752. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Cre mediated recombination then removed exon 2 creating the global null allele. (J:208888)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Panx1 Mutation: 39 strains or lines available

References

• Original: J:208888 Poon IK, et al., Unexpected link between an antibiotic, pannexin channels and apoptosis. Nature. 2014 Mar 20;507(7492):329-34
• All: 11 reference(s)