Tg(tetO-Nog,-EGFP)53Hda
Transgene Detail

Summary

• Symbol: Tg(tetO-Nog,-EGFP)53Hda
• Name: transgene insertion 53, K Sue O'Shea
• MGI ID: MGI:5609523
• Synonyms: pBi-Noggin-EGFP
• Transgene: Tg(tetO-Nog,-EGFP)53Hda Location: unknown Genetic Position: Chr16, Syntenic
• Alliance: Tg(tetO-Nog,-EGFP)53Hda page

Transgene origin

• Strain of Origin: FVB/N-Tg(Nes-rtTA)306Rvs/J

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-Nog,-EGFP)53Hda involves 1 genes/genome features (Golgb1) View all
• Tg(tetO-Nog,-EGFP)53Hda expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Nog (MGI:104327)

• Mutation details: The transgene has a P_bi-1 bi-directional tetracycline-responsive promoter with an enhanced green fluorescent protein-coding region (eGFP) on one side, and a mouse noggin cDNA sequence (encompassing the complete coding region) on the other side. The promoter contains the Tet-responsive element (TRE) placed between two constitutively active, minimal human cytomegalovirus promoters. The eGFP is followed by a beta-globin polyA signal, and the noggin cDNA is followed by an SV40 polyA signal. Analysis at The Jackson Laboratory revealed two complex transgene integration sites (including one transgene-transgene fusion event) both within the Golgb1 locus on chromosome 16. This two duplications of Golgb1 genome region (totaling ~22.3 kb) are fused to transgene sequences (chr16:36,898,704-36,921,071 [mm10]) and chr16:36,899,753-36,899,756 [mm10]). The number of transgene copies at each site was not determined. The transgene insertion effect on Golgb1 expression has not been specifically determined. (J:216792)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:216792 Morell M, et al., Inducible expression of noggin selectively expands neural progenitors in the adult SVZ. Stem Cell Res. 2014 Nov 22;14(1):79-94
• All: 1 reference(s)