Mir22^tm1.2Dzw
Targeted Allele Detail

Summary

• Symbol: Mir22^tm1.2Dzw
• Name: microRNA 22; targeted mutation 1.1, Da-Zhi Wang
• MGI ID: MGI:5585450
• Synonyms: MiR-22^-
• Gene: Mir22 Location: Chr11:75354542-75354636 bp, + strand Genetic Position: Chr11, 45.91 cM
• Alliance: Mir22^tm1.2Dzw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213308
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2 was flanked by loxP sites and a positive-selection neomycin cassette flanked by FRT sites was inserted downstream of exon 2. The neomycin cassette was excised by FLP-mediated recombination. The floxed exon 2 from Mir22^tm1.1Dzw was removed via Cre-mediated recombination leaving a single loxP in place of exon 2. Northern blot and quantitative real-time PCR analysis confirmed absence of expression. (J:213308)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir22 Mutation: 6 strains or lines available

References

• Original: J:213308 Huang ZP, et al., MicroRNA-22 regulates cardiac hypertrophy and remodeling in response to stress. Circ Res. 2013 Apr 26;112(9):1234-43
• All: 3 reference(s)