Klk7^em1Rase
Endonuclease-mediated Allele Detail

Summary

• Symbol: Klk7^em1Rase
• Name: kallikrein related-peptidase 7 (chymotryptic, stratum corneum); endonuclease-mediated mutation 1, Radislav Sedlacek
• MGI ID: MGI:5585278
• Gene: Klk7 Location: Chr7:43460718-43465783 bp, + strand Genetic Position: Chr7, 28.27 cM, cytoband B2
• Alliance: Klk7^em1Rase page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 3 was targeted with TALENs. The DNA binding domain of the 5' TALEN targeted GTAAAGAAGGCTCGC, that of the 3' TALEN targeted CTGCTCAAAGGCAATC. Of the four founder lines, founder 'Klk7 ex3-A' was found to have a 20 bp deletion in exon 3 and was selected for further breeding. Western blots confirmed the absence of peptides translated from this allele. The purpose of this allele was to create a double mutant with Klk5. But because the two genes are too close together on the genome for recombination through cross breeding to work efficiently, the TALEN components were directly injected into oocytes carrying the Klk5^tm1Rase knock-out allele. (J:238698)

Expression

In Mice Carrying this Mutation:

39 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Klk7 Mutation: 13 strains or lines available

References

• Original: J:238698 Kasparek P, et al., KLK5 and KLK7 Ablation Fully Rescues Lethality of Netherton Syndrome-Like Phenotype. PLoS Genet. 2017 Jan;13(1):e1006566
• All: 1 reference(s)