Sec24dtm1d(EUCOMM)Hmgu
Targeted Allele Detail
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Symbol: |
Sec24dtm1d(EUCOMM)Hmgu |
Name: |
SEC24 homolog D, COPII coat complex component; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH |
MGI ID: |
MGI:5582287 |
Gene: |
Sec24d Location: Chr3:123061104-123159290 bp, + strand Genetic Position: Chr3, 53.82 cM, cytoband G3
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Alliance: |
Sec24dtm1d(EUCOMM)Hmgu page
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IMPC: |
Sec24d gene page |
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Mutant Cell Line: |
HEPD0753_7_H04 |
Germline Transmission: |
Earliest citation of germline transmission:
J:101977
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Intragenic deletion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 123086679 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 123088075. The critical exon(s) is/are thus flanked by loxP sites. The lacZ and neomycin segments were excised with germline Flp recombinase, leaving exons 5 and 6 flanked by loxP sites. Then animals were crossed with a germline EIIa-Cre strain to delete exons 5 and 6. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.
(J:157065)
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Original: |
J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015; |
All: |
2 reference(s) |
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