Hprt1^{tm4(CAG-Tbx18/VP64,-Venus)Akis}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm4(CAG-Tbx18/VP64,-Venus)Akis}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 4, Andreas Kispert
• MGI ID: MGI:5576943
• Synonyms: Hprt1^{tm4(CAG-Tbx18*,-Venus)Akis}, Hprt^Tbx18VP16
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm4(CAG-Tbx18/VP64,-Venus)Akis} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:210076
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Hprt1^{tm4(CAG-Tbx18/VP64,-Venus)Akis} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tbx18 (MGI:1923615)

• Mutation details: The targeting construct inserted into the locus a CAG promoter, loxP site, neomycin resistance/STOP cassette, loxM site, and in an inverted cassette containing loxP and loxM sites, Tbx18 cDNA with a VP16 activator construct (VP64), IRES, Venus reporter sequence and polyadenylation sequence. Cre-mediated recombination is required to achieve expression of the inverted cassette. (J:210076)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:210076 Greulich F, et al., Tbx18 function in epicardial development. Cardiovasc Res. 2012 Dec 1;96(3):476-83
• All: 2 reference(s)