Dcbld2^tm1.1Mhms
Targeted Allele Detail

Summary

• Symbol: Dcbld2^tm1.1Mhms
• Name: discoidin, CUB and LCCL domain containing 2; targeted mutation 1.1, Mehran M Sadeghi
• MGI ID: MGI:5569304
• Synonyms: Esdn^-
• Gene: Dcbld2 Location: Chr16:58228806-58290090 bp, + strand Genetic Position: Chr16, 34.52 cM, cytoband C1.1
• Alliance: Dcbld2^tm1.1Mhms page

Attenuation of adult angiogenesis following left femoral artery ligation in Dcbld2^tm1.1Mhms/Dcbld2^tm1.1Mhms mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207629
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A loxP site was inserted upstream of exon 1. A floxed neomycin resistance cassette was inserted downstream of exon 1. Cre-mediated recombination removed exon 1 and the selection cassette. Western blot analysis and flow cytometry confirmed the absence of protein expression in murine lung endothelial cells. (J:207629)

Generation of the Dcbld2^tm1Mhms and Dcbld2^tm1.1Mhms alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dcbld2 Mutation: 47 strains or lines available

References

• Original: J:207629 Nie L, et al., Transmembrane protein ESDN promotes endothelial VEGF signaling and regulates angiogenesis. J Clin Invest. 2013 Dec 2;123(12):5082-97
• All: 3 reference(s)