Igh-J^{tm2.1(4E10)Lave}
Targeted Allele Detail

Summary

• Symbol: Igh-J^{tm2.1(4E10)Lave}
• Name: immunoglobulin heavy chain, joining region; targeted mutation 2.1, Laurent Verkoczy
• MGI ID: MGI:5550246
• Synonyms: 4E10 VH⁺
• Gene: Igh-J Location: unknown Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205708
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The J1-4 regions were replaced with a J558 H10 family promoter, the H10 split leader sequence, a rearranged human heavy chain sequence of the HIV-1 antibody 4E10 (a self-/polyreactive, anti-gp41 BnAb) and a floxed neomycin resistance (neo) cassette. Cre-mediated recombination removed the neo cassette. (J:205708)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh-J Mutation: 12 strains or lines available

References

• Original: J:205708 Chen Y, et al., Common tolerance mechanisms, but distinct cross-reactivities associated with gp41 and lipids, limit production of HIV-1 broad neutralizing antibodies 2F5 and 4E10. J Immunol. 2013 Aug 1;191(3):1260-75
• All: 1 reference(s)