Afmid^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Afmid^{tm1b(EUCOMM)Wtsi}
• Name: arylformamidase; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5548841
• Gene: Afmid Location: Chr11:117716750-117730734 bp, + strand Genetic Position: Chr11, 82.96 cM, cytoband E2
• Alliance: Afmid^{tm1b(EUCOMM)Wtsi} page
• IMPC: Afmid gene page

Mutation origin

• Mutant Cell Line: EPD0155_6_A07
• Germline Transmission: Earliest citation of germline transmission: J:204739
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: Cre-mediated excision of the parental Afmid^{tm1a(EUCOMM)Wtsi} allele resulted in the removal of the promoter-driven neomycin selection cassette and the critical exon 2 leaving behind the inserted lacZ reporter sequence. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. qRT-PCR on liver and kidney confirmed absence of RNA in homozygotes. RNA expression of the immediately adjacent Tk1 gene is reduced by 52% in the kidney and 94% in liver of homozygotes. (J:204739, J:226537)

Expression

In Mice Carrying this Mutation:	11 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Afmid Mutation: 38 strains or lines available

References

• Original: J:204739 International Knockout Mouse Consortium, MGI download of modified allele data from IKMC and creation of new knockout alleles. Database Download. 2014-2023
• All: 7 reference(s)