Sema4a^tm3.1Akum
Targeted Allele Detail

Summary

• Symbol: Sema4a^tm3.1Akum
• Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A; targeted mutation 3.1, Atsushi Kumanogoh
• MGI ID: MGI:5548203
• Synonyms: Sema4A^F350C
• Gene: Sema4a Location: Chr3:88343266-88368489 bp, - strand Genetic Position: Chr3, 38.83 cM
• Alliance: Sema4a^tm3.1Akum page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205754
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 2 and 3 were replaced with a full-length cDNA containing nucleotide substitutions that result in the amino acid substitution of cysteine for phenylalanine at position 350 (F350C) fused to EGFP and followed by a floxed neomycin ressitance cassette. Flp-mediated recombination removed the selection cassette. (J:205754)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sema4a Mutation: 41 strains or lines available

References

• Original: J:205754 Nojima S, et al., A point mutation in Semaphorin 4A associates with defective endosomal sorting and causes retinal degeneration. Nat Commun. 2013;4:1406
• All: 2 reference(s)