Gt(ROSA)26Sor^{tm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, David H Gutmann
• MGI ID: MGI:5543802
• Synonyms: LSL-f-BRAF, LSL-KIAA1549:BRAF
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204469
• Parent Cell Line: EDJ22 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter, Transactivator)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm} expresses 2 genes
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  BRAF (673)

  Braf	(MGI:88190)

  human

  KIAA1549 (57670)

  D630045J12Rik

  (MGI:2669829)

• Mutation details: The targeting vector introduced into the locus a floxed neomycin resistance/STOP cassette, tetracycline-regulated transcription activator (tTA), insulator, hCMV*1 promoter, intron, fusion of human KIAA1549 (exons 1 to 16) and BRAF (exons 9 to 18) (KIAA:BRAF 16_9), IRES and EGFP. This fusion was found in pilocytic astrocytomas of young children. (J:204469)

Generation of the Gt(ROSA)26Sor^{tm2(tTA,CMV*1-KIAA1549/BRAF,-EGFP)Gtm} allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1046 strains or lines available

References

• Original: J:204469 Kaul A, et al., Conditional KIAA1549:BRAF mice reveal brain region- and cell type-specific effects. Genesis. 2013 Oct;51(10):708-16
• All: 2 reference(s)