Nkx2-2tm4.1(cre/EGFP)Suss
Targeted Allele Detail
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Symbol: |
Nkx2-2tm4.1(cre/EGFP)Suss |
Name: |
NK2 homeobox 2; targeted mutation 4.1, Lori Sussel |
MGI ID: |
MGI:5538250 |
Synonyms: |
Nkx2.2Cre, Nkx2.2:Cre-EGFP |
Gene: |
Nkx2-2 Location: Chr2:147019466-147036163 bp, - strand Genetic Position: Chr2, 72.63 cM, cytoband H
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Alliance: |
Nkx2-2tm4.1(cre/EGFP)Suss page
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Germline Transmission: |
Earliest citation of germline transmission:
J:204532
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Parent Cell Line: |
TL1/TL-1 (ES Cell)
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Strain of Origin: |
129S6/SvEvTac
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Allele Type: |
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Targeted (Null/knockout, Recombinase) |
Mutations: |
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Insertion, Intragenic deletion
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Nkx2-2tm4.1(cre/EGFP)Suss expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Nkx2-2 (MGI:97347) |
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Mutation details: A cre/EGFP fusion gene and Frt-flanked hygro selection cassette were inserted using recombineering into an Nkx2-2 basal exchange vector such that cre/EGFP was fused in-frame with the ATG start codon, replacing all but 25 base pairs of the first coding exon. This vector was co-injected with a cre-expression vector into ES cells containing an Nkx2-2 (loxed cassette acceptor) allele. Correctly exchanged ES clones were used to produce chimeras which were bred to B6 mice to verify germline transmission. Animals were bred to ACTB-Flpe mice to excise the hygromycin marker.
(J:204532)
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Generation of Nkx2-2tm4.1(cre/EGFP)Suss. |
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Nkx2-2
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:204532 Balderes DA, et al., Nkx2.2:Cre knock-in mouse line: A novel tool for pancreas- and CNS-specific gene deletion. Genesis. 2013 Dec;51(12):844-51 |
All: |
8 reference(s) |
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