Braf^tm1.1Brd
Targeted Allele Detail

Summary

• Symbol: Braf^tm1.1Brd
• Name: Braf transforming gene; targeted mutation 1.1, Allan Bradley
• MGI ID: MGI:5527388
• Synonyms: Braf^V637E
• Gene: Braf Location: Chr6:39580171-39702397 bp, - strand Genetic Position: Chr6, 18.43 cM
• Alliance: Braf^tm1.1Brd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199307
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Single point mutation
• Mutation details: A lox2272 flanked polyadenylation signal was further enclosed in "Sleeping Beauty" inverted terminal repeats. This structure was inserted in intron 17 and followed by an FRT flanked puromycin resistance cassette. In addition, a T1910A point mutation modified codon 637 in exon 18 so that it encoded glutamic acid rather than valine (p.V637E), recapitulating the human p.V600E mutation. Properly targeted males were mated to females ubiquitously expressing flp recombinase in order to remove the selection cassette. The remaining STOP sequence prevents allele expression but can be removed by cre expression. (J:199307)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Braf Mutation: 58 strains or lines available

References

• Original: J:199307 Rad R, et al., A Genetic Progression Model of Braf(V600E)-Induced Intestinal Tumorigenesis Reveals Targets for Therapeutic Intervention. Cancer Cell. 2013 Jul 8;24(1):15-29
• All: 1 reference(s)