Lin28b^tm1.1Gqda
Targeted Allele Detail

Summary

• Symbol: Lin28b^tm1.1Gqda
• Name: lin-28 homolog B; targeted mutation 1.1, George Q Daley
• MGI ID: MGI:5519055
• Gene: Lin28b Location: Chr10:45252713-45362410 bp, - strand Genetic Position: Chr10, 23.5 cM
• Alliance: Lin28b^tm1.1Gqda page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199720
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 2. Cre-mediated recombination removed exon 2. Western blot analysis confirmed the absence of protein expression in embryonic stem cells. (J:199720)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lin28b Mutation: 22 strains or lines available

References

• Original: J:199720 Shinoda G, et al., Fetal deficiency of lin28 programs life-long aberrations in growth and glucose metabolism. Stem Cells. 2013 Aug;31(8):1563-73
• All: 6 reference(s)