Itm2a^tm1.2Buck
Targeted Allele Detail

Summary

• Symbol: Itm2a^tm1.2Buck
• Name: integral membrane protein 2A; targeted mutation 1.2, Margaret E Buckingham
• MGI ID: MGI:5518653
• Synonyms: Itm2a^KO-IRESnacZ, Itm2a^XKO
• Gene: Itm2a Location: ChrX:106440801-106446966 bp, - strand Genetic Position: ChrX, 47.52 cM, cytoband A2-A3
• Alliance: Itm2a^tm1.2Buck page

Phenotypic analysis of Itm2a mutant embryos

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:200546
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Mutation details: A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 4. An IRES-nlacZ was inserted into exon 6. Flp-mediated recombination removed the neomycin resistance cassette. Cre-mediated recombination removed exons 2 through 4 floxed but left the lacZ reporter intact. In situ hybridization confirmed reduced transcript expression in E11.5 embryos. (J:200546)

Generation of the Itm2a^tm1Buck, Itm2a^tm1.1Buck and Itm2a^tm1.2Buck alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itm2a Mutation: 5 strains or lines available

References

• Original: J:200546 Lagha M, et al., Itm2a is a Pax3 target gene, expressed at sites of skeletal muscle formation in vivo. PLoS One. 2013;8(5):e63143
• All: 1 reference(s)