About   Help   FAQ
Transgene Detail
Symbol: Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey
Name: transgene insertion 17.96, Claude-Agnes Reynaud
MGI ID: MGI:5505386
Synonyms: Lkappa(TG)11, Lk(TG)11
Transgene: Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey  Location: unknown  
Alliance: Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey page
Strain of Origin:  (C57BL/6 x DBA/2)F2
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey expresses 1 gene
Mutation detailsThis transgene comprises two copies of a modified version of an immunoglobulin kappa light chain (Igk) construct containing the rearranged mouse 2-phenyl-oxazolone (phOx) specific VkOx1-Jk5 variable region, the mouse intronic Igk enhancer, the rat Igk constant region (Igk-C) and 8 kb of mouse 3'-flanking sequence including the 3' enhancer. The modification consists of the introduction of two eleven-TG dinucleotide tracts, one at the border of complementarity determining region 1 (CDR1) and framework region 2 (FR2) of the variable region and the second at the beginning of Igk-C. These insertions cause a frameshift that creates a stop codon at the beginning of CDR2, so the gene as passed through the germline is non-functional. It undergoes mutation in the B-cell lineage, at least in Peyer's patches. (J:199560)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:199560 Bertocci B, et al., Probing immunoglobulin gene hypermutation with microsatellites suggests a nonreplicative short patch DNA synthesis process. Immunity. 1998 Aug;9(2):257-65
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
MGI 6.24
The Jackson Laboratory