Igk^{tm2.1(4E10)Nemz}
Targeted Allele Detail

Summary

• Symbol: Igk^{tm2.1(4E10)Nemz}
• Name: immunoglobulin kappa chain complex; targeted mutation 2.1, David Nemazee
• MGI ID: MGI:5505303
• Synonyms: 4E10L
• Gene: Igk Location: Chr6:67532620-70703738 bp, + strand Genetic Position: Chr6, 30.89 cM, cytoband F1-pter

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205764
• Parent Cell Line: iTL IC1 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A floxed neomycin resistance cassette replaced the J cluster in the Igk gene with the HIV antibody 4E10 L chain VJ element. Cre-mediated recombination removed the neomycin resistance cassette. (J:205764)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igk Mutation: 25 strains or lines available

References

• Original: J:205764 Doyle-Cooper C, et al., Immune tolerance negatively regulates B cells in knock-in mice expressing broadly neutralizing HIV antibody 4E10. J Immunol. 2013 Sep 15;191(6):3186-91
• All: 2 reference(s)