Nras^tm2.1Emfu
Targeted Allele Detail

Summary

• Symbol: Nras^tm2.1Emfu
• Name: Nras proto-oncogene, GTPase; targeted mutation 2.1, Ernst-Martin Fuchtbauer
• MGI ID: MGI:5502420
• Synonyms: LTR9NAS
• Gene: Nras Location: Chr3:102965643-102975230 bp, + strand Genetic Position: Chr3, 45.25 cM
• Alliance: Nras^tm2.1Emfu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199340
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

Allele Type: Targeted (Not Applicable) Mutation: Insertion Mutation details: A floxed PGK/Tn5 neomycin resistance (neo) cassette and the Akv1-99 murine leukemia virus long terminal repeat were inserted into intron 1 via homologous recombination. The viral long terminal repeat was inserted at the proviral integration site 9 in the anti-sense direction. Cre-mediated recombination removed the neo cassette. Western blot analysis confirmed increased protein expression in the spleen and thymus. (J:199340)

Schematic for the generation of the Nras^tm1.1Emfu, Nras^tm2.1Emfu, Nras^tm3Emfu, and Nras^tm4Emfu alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nras Mutation: 40 strains or lines available

References

• Original: J:199340 Ballarin-Gonzalez B, et al., Deregulated Nras expression in knock-in animals harboring a gammaretroviral long terminal repeat at the Nras/Csde1 locus. PLoS One. 2013;8(2):e56029
• All: 1 reference(s)