Epx^{tm1.1(cre)Jlee}
Targeted Allele Detail

Summary

• Symbol: Epx^{tm1.1(cre)Jlee}
• Name: eosinophil peroxidase; targeted mutation 1.1, James J Lee
• MGI ID: MGI:5498555
• Synonyms: eoCRE
• Gene: Epx Location: Chr11:87754826-87766362 bp, - strand Genetic Position: Chr11, 52.25 cM
• Alliance: Epx^{tm1.1(cre)Jlee} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198489
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Epx^{tm1.1(cre)Jlee} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Epx (MGI:107569)

• Mutation details: A sequence encoding a mammalian codon-optimized cre recombinase was inserted at the start codon of the Epx locus by homologous recombination. An IRES was inserted before the coding exons of Epx, so that a dicstronic mRNA encoding cre and Epx would be generated. A neo selection cassette was included in the original construct, and was subsequently removed from the targeted allele by a backcross to a flp deleter mouse strain. It was determined that Epx expression was not rescued efficienty from the modified allele by the IRES. Flow cytometric GFP analyses of cell populations from offspring of eoCre mice and reporter animals demonstrated that cre expression is observed only in eosinophils. (J:198489)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Epx (mouse) Summary of all recombinase alleles driven by Epx. Comparative matrix view of recombinase activities Alliance mouse Epx gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Epx Mutation: 34 strains or lines available

References

• Original: J:198489 Doyle AD, et al., Homologous recombination into the eosinophil peroxidase locus generates a strain of mice expressing Cre recombinase exclusively in eosinophils. J Leukoc Biol. 2013 Jul;94(1):17-24
• All: 21 reference(s)