Brca1^tm3.1Rjbr
Targeted Allele Detail

Summary

• Symbol: Brca1^tm3.1Rjbr
• Name: breast cancer 1, early onset; targeted mutation 3.1, Richard J Baer
• MGI ID: MGI:5494435
• Synonyms: Brca1^FH-I26A
• Gene: Brca1 Location: Chr11:101379590-101442781 bp, - strand Genetic Position: Chr11, 65.18 cM, cytoband D
• Alliance: Brca1^tm3.1Rjbr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177853
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 2 was replaced with a modified exon 2 containing an artificial initiator methionine, FLAG and HA epitope tags and nucleotide substitutions that result in the amino acid substitution of alanine for isoleucine at position 26 (I26A). This mutation results in an E3 ligase enzymatically defective protein. Flp-mediated recombination removed a FRT-flanked hygromycin resistance cassette inserted upstream of the modified exon 2. (J:177853, J:197744)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Brca1 Mutation: 113 strains or lines available

References

• Original: J:177853 Shakya R, et al., BRCA1 tumor suppression depends on BRCT phosphoprotein binding, but not its E3 ligase activity. Science. 2011 Oct 28;334(6055):525-8
• All: 3 reference(s)