Rax^{tm1.1(rTA,tetO-cre)Lan}
Targeted Allele Detail

Summary

• Symbol: Rax^{tm1.1(rTA,tetO-cre)Lan}
• Name: retina and anterior neural fold homeobox; targeted mutation 1.1, Richard A Lang
• MGI ID: MGI:5494255
• Synonyms: Rx-tTA:TetOp-Cre
• Gene: Rax Location: Chr18:66067710-66072160 bp, - strand Genetic Position: Chr18, 39.03 cM
• Alliance: Rax^{tm1.1(rTA,tetO-cre)Lan} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:195568
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Rax^{tm1.1(rTA,tetO-cre)Lan} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Rax (MGI:109632)

• Mutation details: A cassette encoding an IRES-tTA (tetracycline transactivator) and tetO-cre was inserted into the 3' UTR of the Rax (Rx) gene, 104 bp from the stop codon. A neo resistance marker was included in the cassette. The construct was electroporated into ES cells for homologous recombination. Correctly targeted germline mutants were crossed to flpe-expressing transgenic mice to remove the neo marker. Further breeding to wild-type mice removed the flpe transgene. Removal of doxycycline from the animals' diets permits Rax-dependent cre expression. (J:195568)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Rax (mouse) Summary of all recombinase alleles driven by Rax. Alliance mouse Rax gene page

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rax Mutation: 14 strains or lines available

Notes

ES cells = 129Sv-derived

References

• Original: J:195568 Plageman TF Jr, et al., Generation of an Rx-tTA: TetOp-Cre knock-in mouse line for doxycycline regulated Cre activity in the Rx expression domain. PLoS One. 2012;7(11):e50426
• All: 1 reference(s)