Ghrltm2.1(cre/EGFP)Suss
Targeted Allele Detail
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Symbol: |
Ghrltm2.1(cre/EGFP)Suss |
Name: |
ghrelin; targeted mutation 2.1, Lori Sussel |
MGI ID: |
MGI:5485215 |
Synonyms: |
Ghrl-Cre, grehlinCre-EGFP |
Gene: |
Ghrl Location: Chr6:113693080-113696841 bp, - strand Genetic Position: Chr6, 52.84 cM, cytoband E3
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Alliance: |
Ghrltm2.1(cre/EGFP)Suss page
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Germline Transmission: |
Earliest citation of germline transmission:
J:195663
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
Not Specified
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Allele Type: |
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Targeted (RMCE-ready, Recombinase, Reporter) |
Mutations: |
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Insertion, Intragenic deletion
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Ghrltm2.1(cre/EGFP)Suss expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: A vector for recombinase-mediated cassette exchange contained the with the sequence encoding a cre/EGFP fusion protein was inserted into a vector containing the region of Ghrl (from upstream of exon 1 to downstream of exon 5) that had been replaced to make the Ghrltm1Suss LCA allele. The cre/EGFP open reading frame was inserted at the endogenous start codon. The vector was coinjected with a cre expressing plasmid into ES cells carrying the Ghrltm1Suss allele, and the selection cassettes in the LCA allele were replaced with the modified Ghrl sequence. ES cells were injected into blastocysts to generate chimeras that were bred to wild-type Black Swiss mice to obtain germline transmission. These mice were crossed to Tg(ACTBFLPe)9205Dym mice to remove the hygro marker included with the cre/EGFP sequence.
(J:195663)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Ghrl
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:195663 Arnes L, et al., Ghrelin expression in the mouse pancreas defines a unique multipotent progenitor population. PLoS One. 2012;7(12):e52026 |
All: |
3 reference(s) |
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