Gsk3a^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gsk3a^{tm1c(EUCOMM)Wtsi}
• Name: glycogen synthase kinase 3 alpha; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5476700
• Synonyms: GSK-3alpha^fl
• Gene: Gsk3a Location: Chr7:24927683-24937276 bp, - strand Genetic Position: Chr7, 13.73 cM
• Alliance: Gsk3a^{tm1c(EUCOMM)Wtsi} page
• IMPC: Gsk3a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:195008
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt1 cassette was inserted at position 24934747 of Chromosome 7 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 2 at position 24935591. The critical exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression. Western blot analysis confirmed normal protein expression levels in the kidney. (J:195008)

Expression

In Mice Carrying this Mutation:

4 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gsk3a Mutation: 18 strains or lines available

References

• Original: J:195008 Barrell WB, et al., Novel reporter alleles of GSK-3alpha and GSK-3beta. PLoS One. 2012;7(11):e50422
• All: 1 reference(s)