Dclk1^{tm1.1(cre/ERT2)Seno}
Targeted Allele Detail

Summary

• Symbol: Dclk1^{tm1.1(cre/ERT2)Seno}
• Name: doublecortin-like kinase 1; targeted mutation 1.1, Hiroshi Seno
• MGI ID: MGI:5475100
• Synonyms: Dclk1^CreERT2
• Gene: Dclk1 Location: Chr3:55149785-55446489 bp, + strand Genetic Position: Chr3, 26.53 cM, cytoband D
• Alliance: Dclk1^{tm1.1(cre/ERT2)Seno} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:193873
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Dclk1^{tm1.1(cre/ERT2)Seno} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Dclk1 (MGI:1330861)

• Mutation details: Homologous recombination was employed to insert a cre/ERT2-IRES-EGFP-polyA-Frt-neo-Frt cassette into the Dclk1 gene immediately after the ATG start codon in exon 2. After germline transmission was established, the neo cassette was excised in vivo by crossing mutants to Flp-expressing mice. (J:193873)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Dclk1 (mouse) Summary of all recombinase alleles driven by Dclk1. Alliance mouse Dclk1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dclk1 Mutation: 53 strains or lines available

References

• Original: J:193873 Nakanishi Y, et al., Dclk1 distinguishes between tumor and normal stem cells in the intestine. Nat Genet. 2013 Jan;45(1):98-103
• All: 6 reference(s)