Nrxn1^tm3Sud
Targeted Allele Detail

Summary

• Symbol: Nrxn1^tm3Sud
• Name: neurexin I; targeted mutation 3, Thomas C Sudhof
• MGI ID: MGI:5468258
• Synonyms: LV1-Nrxn1-HA-tagged
• Gene: Nrxn1 Location: Chr17:90341059-91400499 bp, - strand Genetic Position: Chr17, 59.73 cM
• Alliance: Nrxn1^tm3Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:279669
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Exon 24, the last coding exon of the gene, encodes the transmembrane region of the protein as well as the cytoplasmic C-terminal. A 2xHA (hemagglutinin) tag followed by a loxP site was inserted in-frame into exon 24. A second loxP site was placed after the stop codon, immediately followed by an FRT-flanked PGK-neomycin resistance cassette. (J:279669)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nrxn1 Mutation: 95 strains or lines available

References

• Original: J:279669 Trotter JH, et al., Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters. J Cell Biol. 2019 Aug 5;218(8):2677-2698
• All: 6 reference(s)