Vtcn1^tm1Xxz
Targeted Allele Detail

Summary

• Symbol: Vtcn1^tm1Xxz
• Name: V-set domain containing T cell activation inhibitor 1; targeted mutation 1, Xingxing Zang
• MGI ID: MGI:5463743
• Synonyms: B7x^-
• Gene: Vtcn1 Location: Chr3:100732775-100804238 bp, + strand Genetic Position: Chr3, 43.78 cM
• Alliance: Vtcn1^tm1Xxz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177612
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 2.5 kb fragment of exon 3 and part of intron 3 and a 5.2 kb containing exon 5, intron 5 and exon 6 were cloned into XhoI and salI sites of target vector pKO (Lexicon). PGK promoter-derived Neomycin phosphotransferase cDNA (positive selection) and Diphtheria toxin A china cDNA (negative selection) were cloned into AscI and RsrII sites of the vector. (J:177612)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vtcn1 Mutation: 22 strains or lines available

References

• Original: J:177612 Wei J, et al., Tissue-specific expression of B7x protects from CD4 T cell-mediated autoimmunity. J Exp Med. 2011 Aug 1;208(8):1683-94
• All: 4 reference(s)