Syk^{tm2.1(ZAP70)Fkfr}
Targeted Allele Detail

Summary

• Symbol: Syk^{tm2.1(ZAP70)Fkfr}
• Name: spleen tyrosine kinase; targeted mutation 2.1, Friedemann Kiefer
• MGI ID: MGI:5442724
• Synonyms: Syk^ki, Syk^Zap-70, Syk^{Zap-70 delta}
• Gene: Syk Location: Chr13:52737209-52802828 bp, + strand Genetic Position: Chr13, 27.41 cM, cytoband B-C2
• Alliance: Syk^{tm2.1(ZAP70)Fkfr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:188422
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Syk^{tm2.1(ZAP70)Fkfr} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ZAP70 (7535)

  Zap70

  (MGI:99613)

• Mutation details: The coding sequence in exon 2 was replaced with a human cDNA for Zap70 and an FRT-flanked neo cassette. Flp-mediated recombination removed the neo cassette. Western blot analysis confirmed the absence of endogenous protein expression and expression of the human cDNA. (J:188422)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Syk Mutation: 42 strains or lines available

References

• Original: J:188422 Konigsberger S, et al., Altered BCR signalling quality predisposes to autoimmune disease and a pre-diabetic state. EMBO J. 2012 Aug 1;31(15):3363-74
• All: 3 reference(s)