Ccm2l^tm1Mlkn
Targeted Allele Detail

Summary

• Symbol: Ccm2l^tm1Mlkn
• Name: cerebral cavernous malformation 2-like; targeted mutation 1, Mark L Kahn
• MGI ID: MGI:5439489
• Synonyms: Ccm2l^GFP
• Gene: Ccm2l Location: Chr2:152907875-152923655 bp, + strand Genetic Position: Chr2, 75.41 cM
• Alliance: Ccm2l^tm1Mlkn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:187714
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A construct including GFP with a nuclear localization tag and an FRT flanked neo cassette was inserted in frame with the start methionine replacing a genomic fragment including most of exon 1 and all of intron 1 and exon 2. GFP is detected in the nuclei of PECAM+ endocardial and endothelial cells. Quantitative real time PCR and RT-PCR confirmed the absence of full length mRNA expression in tissues from homozygous mice. (J:187714)

Expression

In Mice Carrying this Mutation:

72 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ccm2l Mutation: 14 strains or lines available

References

• Original: J:187714 Zheng X, et al., Dynamic regulation of the cerebral cavernous malformation pathway controls vascular stability and growth. Dev Cell. 2012 Aug 14;23(2):342-55
• All: 1 reference(s)