Trp53^tm2.1Wgu
Targeted Allele Detail

Summary

• Symbol: Trp53^tm2.1Wgu
• Name: transformation related protein 53; targeted mutation 2.1, Wei Gu
• MGI ID: MGI:5431730
• Synonyms: p53^3KR
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm2.1Wgu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186159
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exons 4 and 5 were replaced with modified ones in which nucleotide substitutions results in the amino acid substitutions of lysine with arginine at positions 117, 161 and 162 (p.K117R (c.350_351delAGinsGA), p.K161R and p.K162R (c.482_486delAGAAGinsGGAGA). A loxP site flanked neomycin resistance gene cassette inserted between the two modified exons was removed by Cre-mediated recombination. (J:186159)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 238 strains or lines available

References

• Original: J:186159 Li T, et al., Tumor suppression in the absence of p53-mediated cell-cycle arrest, apoptosis, and senescence. Cell. 2012 Jun 8;149(6):1269-83
• All: 3 reference(s)