Gt(ROSA)26Sor^{tm1(tTA,tetO-Mir155)Fjsl}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(tTA,tetO-Mir155)Fjsl}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Frank J Slack
• MGI ID: MGI:5431136
• Synonyms: Gt(ROSA)26Sor^{tm1(tetO-Mir155)Fjsl}, mir-155^LSLtTA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(tTA,tetO-Mir155)Fjsl} page

Gt(ROSA)26Sor^{tm1(tTA,tetO-Mir155)Fjsl}/Gt(ROSA)26Sor⁺ Tg(Nes-cre)1Wmz/0 mice develop disseminated lymphoma

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:185598
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (C57BL/6J x SJL/J)F2

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Inserted expressed sequence, Transactivator)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(tTA,tetO-Mir155)Fjsl} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Mir155 (MGI:2676840)

• Mutation details: A construct was inserted into the locus that contained the following elements (5' to 3'): the tetracycline transactivator (tTA), two poly-adenylation sequences, the tetracycline operator with minimal CMV promoter, a floxed neo cassette with 4 poly-adenylation sequences, the mouse transcript, and a poly-adenylation sequence. The locus promoter drives expression of tTA while the tet operator drives expression of the transcript only after cre mediated removal of the floxed neo-STOP cassette that can be repressed by doxycycline treatment. (J:185598)

Schematic of the Gt(ROSA)26Sor^{tm1(tTA,tetO-Mir155)Fjsl} allele

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Gt(ROSA)26Sor^{tm1(tTA,tetO-Mir155)Fjsl}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:185598 Babar IA, et al., Nanoparticle-based therapy in an in vivo microRNA-155 (miR-155)-dependent mouse model of lymphoma. Proc Natl Acad Sci U S A. 2012 Jun 26;109(26):E1695-704
• All: 4 reference(s)