Grin1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Grin1^{tm1c(EUCOMM)Wtsi}
• Name: glutamate receptor, ionotropic, NMDA1 (zeta 1); targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5431094
• Synonyms: Grin1^2lox
• Gene: Grin1 Location: Chr2:25181193-25209199 bp, - strand Genetic Position: Chr2, 17.14 cM
• Alliance: Grin1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Grin1 gene page

Mutation origin

• Mutant Cell Lines: EPD0469_5_C11, EPD0469_5_G09
• Germline Transmission: Earliest citation of germline transmission: J:155845
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

Allele Type: Targeted (Conditional ready) Mutation: Insertion Vector: L1L2_Bact_P Mutation details: A targeting vector containing a human beta-actin promoter-driven selection cassette consisting of a FRT site, lacZ sequence, the first loxP site, human beta-actin promoter driving the neomycin resistance gene, SV40 polyA, FRT site and a second loxP site was inserted downstream of exon 6 of the targeted gene, and another loxP site was inserted upstream of exon 6. Flp-mediated recombination removed the lacZ and neo cassette and left exon 6 floxed. (J:155845, J:193625)

Generation of the Grin1^tm1c(EUCOMM)Wtsi allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Grin1 Mutation: 64 strains or lines available

Notes

Cell line EPD0469_5_G09 and EPD0469_5_C11 were successfully used to make chimeric mice. Germline transmission was accomplished. J:155845 J:193625

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 3 reference(s)