Rab38^em3Rkuhn
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rab38^em3Rkuhn
• Name: RAB38, member RAS oncogene family; endonuclease-mediated mutation 3, Ralf Kuhn
• MGI ID: MGI:5430819
• Synonyms: Rab38^delta
• Gene: Rab38 Location: Chr7:88079481-88140780 bp, + strand Genetic Position: Chr7, 49.19 cM
• Alliance: Rab38^em3Rkuhn page

Mutation origin

• Strain of Origin: (FVB/NCrl x C57BL/6J-Rab38^cht)F1

Mutation description

• Allele Type: Endonuclease-mediated
• Mutations: Intragenic deletion, Nucleotide substitutions
• Mutation details: Zinc finger endonuclease mediated homologous recombination introduced nucleotide substitutions to restore the wild-type coding sequence at codon 19 of the spontaneous Rab38 mutant allele. Additionally, non-homologous end joining resulted in a 27 bp deletion that disrupts the ApaLiI restriction endonuclease site at codons 30 and 31. (J:185512)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rab38 Mutation: 15 strains or lines available

Notes

Targeted oocytes were derived from an F1 cross using FVB female mice and Rab38^cht male mice.

References

• Original: J:185512 Meyer M, et al., Modeling disease mutations by gene targeting in one-cell mouse embryos. Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9354-9
• All: 1 reference(s)