Tg(Gucy2g-cre,-EGFP)52Irod
Transgene Detail

Summary

• Symbol: Tg(Gucy2g-cre,-EGFP)52Irod
• Name: transgene insertion 52, Ivan Rodriguez
• MGI ID: MGI:5430756
• Synonyms: GCG-Cre-GFP
• Transgene: Tg(Gucy2g-cre,-EGFP)52Irod Location: unknown
• Alliance: Tg(Gucy2g-cre,-EGFP)52Irod page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Gucy2g-cre,-EGFP)52Irod expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gucy2g (MGI:106025)

• Mutation details: An Frt-flanked zeomycin-puromycin selection cassette was inserted after an IRES-cre-IRES-EGFP sequence to generate a construct for homologous recombination. The construct was inserted after the stop codon of the Gucy2g (GC-G) gene in the bacterial artificial chromosome BAC RP23-345B13. expression of flippase in EL250 bacterial cells removed the selection cassette. The resulting modified BAC construct was injected into fertilized pronuclei to generated transgenic mice. Eight founders were obtained, with seven lines showing transgene expression in the Grueneberg ganglion. Line 52 exhibited robust GFP expression and was selected for further analysis. (J:185650)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gucy2g (mouse) Summary of all recombinase alleles driven by Gucy2g. Alliance mouse Gucy2g gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:185650 Matsuo T, et al., The wiring of Grueneberg ganglion axons is dependent on neuropilin 1. Development. 2012 Aug;139(15):2783-91
• All: 1 reference(s)