Car5a^tm1Sly
Targeted Allele Detail

Summary

• Symbol: Car5a^tm1Sly
• Name: carbonic anhydrase 5a, mitochondrial; targeted mutation 1, William S Sly
• MGI ID: MGI:5425618
• Gene: Car5a Location: Chr8:122642874-122671651 bp, - strand Genetic Position: Chr8, 70.81 cM
• Alliance: Car5a^tm1Sly page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:181790
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: All of exon 3 as well as 150 bp of intron 2 and 3.05 kb of intron 3 were replaced by a PGK-neomycin resistance gene. This removed two of the three zinc binding histidine residues and created a frameshift beginning in exon 4. No protein was detected by Western blot analysis. (J:181790, J:196135)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Car5a Mutation: 15 strains or lines available

References

• Original: J:181790 Price TO, et al., Topiramate treatment protects blood-brain barrier pericytes from hyperglycemia-induced oxidative damage in diabetic mice. Endocrinology. 2012 Jan;153(1):362-72
• All: 2 reference(s)