Cdk2^tm1.1Jro
Targeted Allele Detail

Summary

• Symbol: Cdk2^tm1.1Jro
• Name: cyclin dependent kinase 2; targeted mutation 1.1, James Roberts
• MGI ID: MGI:5319654
• Synonyms: CDK2AF, CDK2^{T14A Y15F}
• Gene: Cdk2 Location: Chr10:128533808-128540900 bp, - strand Genetic Position: Chr10, 77.13 cM, cytoband D3
• Alliance: Cdk2^tm1.1Jro page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:183801
• Parent Cell Line: AK7.1 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details:

  Exon 1 was replaced with one in which nucleotide substitutions resulted in the amino acid substitution of threonine 14 with alanine and tyrosine 15 with phenylalanine (p.T14_Y15delinsAF). Cre-mediated recombination removed a floxed neomycin resistance gene cassette inserted into intron 1. (J:183801)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdk2 Mutation: 16 strains or lines available

References

• Original: J:183801 Zhao H, et al., Loss of cyclin-dependent kinase 2 (CDK2) inhibitory phosphorylation in a CDK2AF knock-in mouse causes misregulation of DNA replication and centrosome duplication. Mol Cell Biol. 2012 Apr;32(8):1421-32
• All: 1 reference(s)