Tg(Wap-tAg)1Gmn
Transgene Detail

Summary

• Symbol: Tg(Wap-tAg)1Gmn
• Name: transgene insertion 1, A Graessmann
• MGI ID: MGI:5313806
• Synonyms: WAP-SVt, WAP-SVt/1
• Transgene: Tg(Wap-tAg)1Gmn Location: unknown
• Alliance: Tg(Wap-tAg)1Gmn page

Transgene origin

• Strain of Origin: NMRI

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The transgene consists of a 1600 bp DNA fragment containing the promoter of the mouse whey acidic protein gene joined to the SV40 early region, which includes the overlapping sequences encoding the SV40 large and small T-antigen genes. So that only the small t antigen is expressed, the 5' splice site of the large T antigen mRNA was modified by site-directed mutagenesis (CTGAG/GTATTT to CTGAA/GTCTT); this alteration entirely suppresses splicing of the transcript encoding the large T antigen without affecting the coding sequence of the small t antigen. RT-PCR and western blot analysis demonstrate high-level expression of the small t antigen mRNA and protein, respectively, in mammary gland epithelial (ME) cells of transgenic female mice during their first pregnancy that continues in the surviving ME cells after lactation ceases. Neither large T antigen mRNA nor protein is detectable in ME cells of these mice. (J:69711)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Three transgenic mouse lines bearing this construct, which the authors designate WAP-SVt/1, WAP-SVt/6 (which both express the transgene at high levels) and WAP-SVt/8 (whose expression is much lower), are described. Most data shown are for mice of line 1 and, for contrast only, line 8. (J:69711)

References

• Original: J:69711 Goetz F, et al., The SV40 small t-antigen prevents mammary gland differentiation and induces breast cancer formation in transgenic mice; truncated large T-antigen molecules harboring the intact p53 and pRb binding region do not have this effect. Oncogene. 2001 Apr 26;20(18):2325-32
• All: 1 reference(s)