Dll4^tm2.1Vlcg
Targeted Allele Detail

Summary

• Symbol: Dll4^tm2.1Vlcg
• Name: delta like canonical Notch ligand 4; targeted mutation 2.1, Velocigene
• MGI ID: MGI:5302387
• Synonyms: Dll4^COIN, Dll4^i3COIN
• Gene: Dll4 Location: Chr2:119156265-119166147 bp, + strand Genetic Position: Chr2, 59.97 cM, cytoband E3
• Alliance: Dll4^tm2.1Vlcg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177797
• Parent Cell Line: F1H4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A conditional by inversion (COIN) intron consisting of a lox66_SA-Egpf-polyA_lox71 was inserted in the antisense orientation into intron 3 via homologous recombination, splitting intron 3 into 2 new exons (631 and 144 bp in length). The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the inserted sequence. In addition, an FRT flanked hygromycin-delta-thymidine kinase cassette was inserted after the COIN intron. Flp-mediated recombination removed the hygromycin cassette. (J:177797)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dll4 Mutation: 26 strains or lines available

References

• Original: J:177797 Lobov IB, et al., The Dll4/Notch pathway controls postangiogenic blood vessel remodeling and regression by modulating vasoconstriction and blood flow. Blood. 2011 Jun 16;117(24):6728-37
• All: 3 reference(s)