Tg(tetO-APPSwInd)Dbo
Transgene Detail

Summary

• Symbol: Tg(tetO-APPSwInd)Dbo
• Name: transgene insertion, David R Borchelt
• MGI ID: MGI:5295154
• Synonyms: TetO-hAPP, Tg(tetO-APP*Swe*Ind)#Dbo
• Transgene: Tg(tetO-APPSwInd)Dbo Location: unknown
• Alliance: Tg(tetO-APPSwInd)Dbo page

Transgene origin

• Strain of Origin: (C57BL/6J x C3H/HeJ)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-APPSwInd)Dbo expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	App (MGI:88059)	The gene encodes a humanized amyloid beta domain and incorporates the Swedish and Indiana mutations.

• Tg(tetO-APPSwInd)Dbo expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: The mouse amyloid beta (A4) precursor protein (APP) sequence was modified to contain a humanized amyloid-beta (Abeta) domain. This mouse/human chimeric APP (mo/huAPP695 or APP695) was mutated to incorporate the Swedish (KM570/571NL) and Indiana (V617F) APP mutations associated with Alzheimer's disease. This APP695swe/ind sequence was placed downstream of the tetracycline-responsive (TRE or tetO) promoter and mouse prion protein exons 1-2. Lines 18, 102, 107, and 885 were established. When mice are crossed with a line expressing a tTA transgene, these mice show the greatest doxycycline sensitivity for transgene suppression. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:80782, J:109829)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:176127 Cheng N, et al., In Vivo Olfactory Model of APP-Induced Neurodegeneration Reveals a Reversible Cell-Autonomous Function. J Neurosci. 2011 Sep 28;31(39):13699-704
• All: 9 reference(s)