Gt(ROSA)26Sor^{tm1(H1/tetO-RNAi:Pion)Pggd}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(H1/tetO-RNAi:Pion)Pggd}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Paul Greengard
• MGI ID: MGI:5294908
• Synonyms: GSAP-RNAi
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(H1/tetO-RNAi:Pion)Pggd} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:163997
• Parent Cell Line: ART4.12 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Inducible, Knockdown, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(H1/tetO-RNAi:Pion)Pggd} involves 1 genes/genome features (Gsap) View all
• Mutation details: The short hairpin RNA (shRNA) against Pion (5'-TCCCGGAACTCCATGATTGACAAATTTCAAGAGAATTTGTCAATCATGGAGTTCCTTTTTA-3') was inserted into the locus via RMCE with Gt(ROSA)26Sor^{tm1(CAG-flpe)Arte}. The shRNA sequence is under the regulation of the tetO operator and a codon optimized tet repressor (itetR) under the control of a CAG promoter. (J:163997)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:163997 He G, et al., Gamma-secretase activating protein is a therapeutic target for Alzheimer's disease. Nature. 2010 Sep 2;467(7311):95-8
• All: 1 reference(s)