Lmx1a^tm1Ics
Targeted Allele Detail

Summary

• Symbol: Lmx1a^tm1Ics
• Name: LIM homeobox transcription factor 1 alpha; targeted mutation 1, Mouse Clinical Institute
• MGI ID: MGI:5287977
• Synonyms: Lmx1a^eGFP
• Gene: Lmx1a Location: Chr1:167516806-167676310 bp, + strand Genetic Position: Chr1, 75.08 cM
• Alliance: Lmx1a^tm1Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:175535
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment, including exons 1and 2, intron 1 and part of intron 2, was replaced with an EGFP gene and a floxed neo cassette via homologous recombination. In heterozygous mice EGFP expression recapitulates endogenous gene expression. Immunohistochemical analysis confirmed the absence of wild-type protein expression in the ventral midbrain of homozygous embryos at E11.5. (J:175535)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lmx1a Mutation: 23 strains or lines available

References

• Original: J:175535 Deng Q, et al., Specific and integrated roles of Lmx1a, Lmx1b and Phox2a in ventral midbrain development. Development. 2011 Aug;138(16):3399-408
• All: 6 reference(s)