Pi4ka^tm2.1Arte
Targeted Allele Detail

Summary

• Symbol: Pi4ka^tm2.1Arte
• Name: phosphatidylinositol 4-kinase alpha; targeted mutation 2.1, TaconicArtemis
• MGI ID: MGI:5008158
• Synonyms: Pi4ka^R1900K
• Gene: Pi4ka Location: Chr16:17098215-17224178 bp, - strand Genetic Position: Chr16, 10.71 cM
• Alliance: Pi4ka^tm2.1Arte page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:172498
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Single point mutation
• Mutation details: A targeting vector was designed to insert a loxP site upstream of exon 48 of the phosphatidylinositol 4-kinase, catalytic, alpha polypeptide (Pi4ka) gene. A human growth hormone polyadenylation signal (hGHpA) and a second loxP site followed by a frt/F3-flanked neomycin (neo) resistance cassette were placed downstream of exon 55 of Pi4ka. Using a recombination-mediated exchange cassette (RMCE), correctly targeted ES cells were then electroporated with a second targeting vector. This second targeting vector replaces the frt/F3-flanked neo cassette with a attB/attP-flanked puromycin (puro) resistance cassette and loxP site followed by a duplicated genomic region containing exons 48 to 55. The puro cassette and the duplicated region were flanked by frt/F3 sites. The duplicated genomic region contained a point mutation in exon 51 resulting in an Arginine to Lysine substitution (R1900K). PhiC31-mediated recombination removed the puro cassette. (J:172498)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pi4ka Mutation: 129 strains or lines available

References

• Original: J:172498 TaconicArtemis, Direct Data Submission 2011/06/17. MGI Direct Data Submission. 2011
• All: 2 reference(s)