Per2^tm2Jt
Targeted Allele Detail

Summary

• Symbol: Per2^tm2Jt
• Name: period circadian clock 2; targeted mutation 2, Joseph S Takahashi
• MGI ID: MGI:4950936
• Synonyms: mPer2^{Luciferase-SV40}, mPER2-LUC-SV40, mPer2::Svluc, Per2::LucSV
• Gene: Per2 Location: Chr1:91343704-91387046 bp, - strand Genetic Position: Chr1, 46.13 cM, cytoband C5
• Alliance: Per2^tm2Jt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171738
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The luciferase gene, followed by SV40 poly(A) signal sequence, and a loxP site flanked neomycin resistance gene cassette, was inserted into exon 23 before the stop codon, in-frame with the targeted gene coding region. The inserted sequence replaces the endogenous 3' UTR. This creates an allele coding for a chimeric peptide of the endogenous protein fused at the C-terminus to the luminescent marker. (J:171738)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Per2 Mutation: 71 strains or lines available

References

• Original: J:171738 Welsh DK, et al., Bioluminescence imaging of individual fibroblasts reveals persistent, independently phased circadian rhythms of clock gene expression. Curr Biol. 2004 Dec 29;14(24):2289-95
• All: 10 reference(s)