Crisp4^tm1.2Mkob
Targeted Allele Detail

Summary

• Symbol: Crisp4^tm1.2Mkob
• Name: cysteine-rich secretory protein 4; targeted mutation 1.2, Moira K O'Bryan
• MGI ID: MGI:4950060
• Gene: Crisp4 Location: Chr1:18185415-18216126 bp, - strand Genetic Position: Chr1, 5.65 cM, cytoband A4
• Alliance: Crisp4^tm1.2Mkob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171346
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A loxP site was inserted into intron 2 and a floxed neo cassette was inserted into intro 3 via homologous recombination. Cre mediated recombination removed the neo cassette and exon 3. Western blot and immunohistochemical analysis confirmed the absence of protein expression in epididymides from homozygous mice. (J:171346)

Generation of the Crisp4^tm1.1Mkob and Crisp4^tm1.2Mkob alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crisp4 Mutation: 23 strains or lines available

References

• Original: J:171346 Gibbs GM, et al., Cysteine-rich secretory protein 4 is an inhibitor of transient receptor potential M8 with a role in establishing sperm function. Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):7034-9
• All: 1 reference(s)