Grina^tm1.1Ldh
Targeted Allele Detail

Summary

• Symbol: Grina^tm1.1Ldh
• Name: glutamate receptor, ionotropic, N-methyl D-aspartate-associated protein 1 (glutamate binding); targeted mutation 1.1, Lynn D Hudson
• MGI ID: MGI:4943769
• Gene: Grina Location: Chr15:76130964-76134104 bp, + strand Genetic Position: Chr15, 35.59 cM, cytoband E1
• Alliance: Grina^tm1.1Ldh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:169916
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The 8 Kb targeting vector was designed to replace a region upstream of exon 2 to downstream of exon 8 with EGFP and a loxP-flanked neomycin (neo) resistance cassette (in opposite transcriptional orientation to the EGFP). The vector also changed the initiation codon at the beginning of exon 2. The neomycin cassette was removed by crossing mice with a transgenic strain expressing Tg(Ella-cre)C5379Lgmd. Northern blot confirmed the absence of mRNA. EGFP expression is detected throughout the brain, and is elevated in the hippocampus. (J:169916)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Grina Mutation: 17 strains or lines available

References

• Original: J:169916 Hudson LD, Generation of a knock-in mutation in Grina expressing green fluorescent protein (GFP). MGI Direct Data Submission. 2011
• All: 4 reference(s)