Mafa^tm1.1Eyc
Targeted Allele Detail

Summary

• Symbol: Mafa^tm1.1Eyc
• Name: MAF bZIP transcription factor A; targeted mutation 1.1, Alain Eychene
• MGI ID: MGI:4868452
• Synonyms: mafA^LacZ
• Gene: Mafa Location: Chr15:75617339-75620077 bp, - strand Genetic Position: Chr15, 34.82 cM
• Alliance: Mafa^tm1.1Eyc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164344
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The loxP flanked segment of Mafa^tm1Eyc, extending from 46 bp upstream of the ATG translation initiation site of the endogenous gene to 23 bp downstream of its polyadenylation signal (and encompassing a FRT-flanked PGK-neo cassette), has been deleted by Cre recombinase. This places the coding sequence for nuclear localized E. coli beta-galactosidase (lacZ), which had followed the 3' loxP site, under transcriptional control of the Mafa regulatory elements. In the developing embryo, cells expressing lacZ were first observed at embryonic day (E)10.5, in the already-aggregated dorsal root ganglia (DRG), where the number of stained cells and intensity of staining increases through E12. Expression is also observed in the trigeminal ganglia from E12.5 and is seen in other parts of the central nervous system, including spinal cord, olfactory bulb, dorsal diencephalon and brain stem. (J:164344)

Expression

In Mice Carrying this Mutation:

76 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mafa Mutation: 20 strains or lines available

References

• Original: J:164344 Lecoin L, et al., MafA transcription factor identifies the early ret-expressing sensory neurons. Dev Neurobiol. 2010 Jun;70(7):485-97
• All: 2 reference(s)