Sst^{tm2.1(cre)Zjh}
Targeted Allele Detail

Summary

• Symbol: Sst^{tm2.1(cre)Zjh}
• Name: somatostatin; targeted mutation 2.1, Z Josh Huang
• MGI ID: MGI:4838416
• Synonyms: SOM-iCre, SOM-IRES-Cre, SSTCre, Sst-iCre, Sst-IRES-Cre
• Gene: Sst Location: Chr16:23708323-23709708 bp, - strand Genetic Position: Chr16, 15.0 cM, cytoband cen-C3
• Alliance: Sst^{tm2.1(cre)Zjh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151755
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1
• Project Collection: Neuroscience Blueprint cre

Mutation description

• Allele Type: Targeted (Knockdown, Recombinase)
• Mutation: Insertion
• Sst^{tm2.1(cre)Zjh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Sst (MGI:98326)

• Mutation details: A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, a polyA sequence, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the somatostatin locus (Sst). This construct was electroporated into C57BL/6;129 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric males were bred with Actin-FLPe females (on a C57BL/6 congenic background) to achieve germline transmission and to remove the neo selection cassette. This mutation results in a dosage-dependent knock-down of endogenous Sst expression J:279254 . (J:151755)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sst (mouse) Summary of all recombinase alleles driven by Sst. Comparative matrix view of recombinase activities Alliance mouse Sst gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Sst Mutation: 29 strains or lines available

References

• Original: J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009
• All: 442 reference(s)