Smo^tm2.1Amc
Targeted Allele Detail

Summary

• Symbol: Smo^tm2.1Amc
• Name: smoothened, frizzled class receptor; targeted mutation 2.1, Andrew P McMahon
• MGI ID: MGI:4834775
• Gene: Smo Location: Chr6:29735502-29761364 bp, + strand Genetic Position: Chr6, 12.36 cM, cytoband A3.3
• Alliance: Smo^tm2.1Amc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:135134
• Parent Cell Line: AV3 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: LoxP sites and an frt-flanked neomycin resistance cassette were inserted flanking the first coding exon. A tau-LacZ gene was also inserted 3' to the neomycin resistance gene. A null allele was generated by crossing the loxP-flanked allele to beta-actin-Cre expressing mice, an allele with germline Cre expression, and subsequently crossing out the Cre transgene by backcrossing to ICR. (J:73071, J:135134)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smo Mutation: 44 strains or lines available

References

• Original: J:135134 Goddeeris MM, et al., Independent requirements for Hedgehog signaling by both the anterior heart field and neural crest cells for outflow tract development. Development. 2007 Apr;134(8):1593-604
• All: 4 reference(s)